Abcam, ab239433, Human Elastin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human Elastin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Elastin with a sensitivity of 44 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 44 pg/mL,
Range:0.19 - 12 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Elastin ELISA Kit ab239433 is a rapid single-wash 90-min ELISA kit to measure Human Elastin in serum and plasma. This SimpleStep ELISA® sensitivity is 44 pg/mL.
How the assay works
Human Elastin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human Elastin. The standard protein in this kit is an N-terminal fragment representing approximately 40% of the full-length mass of mature human Elastin. Native signal was detected in serum, plasma (citrate), and plasma (EDTA) sample types. Spiked protein experiments were used to validate serum, plasma (citrate), and plasma (EDTA) sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (heparin) samples.
Human Elastin ELISA Kit ab239433 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Elastin ELISA Kit ab239433
Human Elastin ELISA Kit ab239433 has been used to study biomarkers of psoriasis and aging
and skin rejuvenation using engineered extracellular vesicles
References:
Lenka Borska et al. 2022, PMID: 35625870,
Yuan Wan et al. 2019, PMID: 31909348
Save your precious samples
Our 384-well Human Elastin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Elastin Antibody Pair - BSA and Azide free
ab256604
- Anti-Elastin antibody [EPR20414-6] - BSA and Azide free (Capture)
ab259455
- Anti-Elastin antibody [EPR20414-34] - BSA and Azide free (Detector)
ab259456
Related and recommended products
Human Elastin ELISA Kit ab239433 is commonly used to study tissue structure and function. Elastin is a component of the extracellular matrix (ECM).
Other ELISA kits to measure the ECM and its role in tissue function include:
- Human Collagen Type I ELISA kit
ab285250
- Human Pro-Collagen I alpha 1 ELISA kit
ab210966
- Total Glycosaminoglycans Assay Kit
ab289842

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C