Abcam, ab239725, Protein Synthesis Assay Kit (Green)

Size: 100Test
Protein Synthesis Assay Kit (ab239725) labels cells undergoing translation with a fluorescent green dye, using a method based on an alkyne-containing, cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Readout on flow cytometer or fluorescence microscope. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay Platform:Flow cytometer, Fluorescence microscope

Product details:
Protein Synthesis Assay Kit (Green) (ab239725) utilizes a novel and robust chemical method based on an alkyne-containing, cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puromycin).
Protein synthesis assay principle
In the protein synthesis assay, cultured cells are incubated with OP-puromycin. Once inside the cell, OP-puromycin stops translation by forming covalent conjugates with nascent polypeptide chains.
After incubation, cells are then fixed with formalin, washed, and permeabilized with detergent.
Truncated polypeptides bound to OP-puromycin are then labelled with a fluorescent dye, in a copper-dependent reaction using a fluorescent-azide.
Cycloheximide, an inhibitor of translation, is used as a negative control. DAPI is used as a blue nuclear counter-stain.
Unlike methionine analogs, OP-puromycin does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture.
Other protein synthesis assay kits
This kit ab239725 stains cells that have undergone protein synthesis with 5(6)-FAM (Fluorescein), which has a green fluorescence. It is designed for analysis by fluorescent microscopy or flow cytometry.
This kit contains identical components to Protein Synthesis Assay Kit (Green, Microplate)
ab273286
, which is designed for use with a fluorometric microplate reader.
Protein Synthesis Kit (Red)
ab235634
stains cells that have undergone protein synthesis with 5(6)-TAMRA (Rhodamine), which has a red fluorescence (Ex//Em 540/580 nm). It is designed for analysis by fluorescent microscopy or flow cytometry.
Other Notes
This product was previously called K459 EZClick™ Biovision Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence. Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C