Abcam, ab239812, Anti-SGK1 antibody [Y238] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SGK1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y238,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239812 is the carrier-free version of
ab32374
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Serum and Glucocorticoid-Regulated Kinase 1 also known as SGK1 is a protein kinase involved in cellular stress response. It consists of approximately 49 kDa and is encoded by the SGK1 gene. SGK1 is expressed in several tissues including the kidney pancreas and liver. It transfers phosphate groups from ATP to serine or threonine residues on target proteins functioning as an important regulator of ion channels enzymes and other cellular proteins.
Biological function summary
SGK1 regulates ion transport cell proliferation and survival pathways. SGK1 is not usually part of large protein complexes but interacts dynamically with other proteins to exert its effects. It plays a major role in regulating sodium channels in the kidney therefore modulating sodium balance and blood pressure. It also affects various cell survival mechanisms influencing cell growth and apoptosis in response to physiological cues.
Pathways
SGK1 is involved in the PI3K/AKT/mTOR signaling pathway and the WNK/SPAK pathway. These pathways influence cellular growth metabolism and ion transport. SGK1 shares pathway relevance with proteins like AKT1 and mTOR in the PI3K/AKT/mTOR axis impacting various metabolic and growth-related cellular processes. In the WNK/SPAK pathway SGK1 regulates ion channels in collaboration with WNK kinases affecting electrolyte balance.
SGK1 has implications in hypertension and cancer. Overactivity of SGK1 can lead to increased sodium retention resulting in hypertension. This connects SGK1 to diseases involving blood pressure regulation where it often interacts with the epithelial sodium channel (ENaC). In cancer SGK1 contributes to tumor progression by supporting cell survival with interactions alongside AKT1 frequently observed in cancerous pathways. Alterations in SGK1 levels or activity may therefore serve as a therapeutic target for managing these conditions.