Abcam, ab239825, Anti-Rho antibody [EP487Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RHOA antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP487Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for Rho. It is also expected to detect RhoA, RhoB and RhoC.

Product details:
ab239825 is the carrier-free version of
ab40673
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rho is a small GTPase known for its role in regulating the cytoskeleton. Alternate names include RhoA RhoB and RhoC. Rho proteins have a molecular mass of about 21 kDa. It is expressed in various tissues with particularly high levels in muscle the brain and fibroblasts. Rho belongs to the Ras homologous (Rho) family of GTPases and acts as a molecular switch that toggles between an active GTP-bound state and an inactive GDP-bound state.
Biological function summary
By controlling the organization of the cytoskeleton Rho plays a vital role in cell shape motility and division. It is part of the Rho family GTPase complex which includes other members like Rac and Cdc42. These proteins coordinate actin filament formation affecting cellular movements such as migration and adhesion. Rho also influences the formation of stress fibers and focal adhesions linking it to mechanical stress response and cell polarity.
Pathways
Rho orchestrates cell activities through pathways like the Rho/Rho kinase (ROCK) and the phosphatidylinositol-45-bisphosphate 3-kinase (PI3K) pathways. In the Rho/ROCK pathway Rho activates ROCK leading to actomyosin contractility and cell movement. Rho also interacts with proteins like Rac and Cdc42 in the PI3K pathway coordinating diverse cellular responses. This involvement is important for processes such as cell cycle progression and apoptosis.
Improper Rho functioning is linked to conditions like cancer and cardiovascular diseases. Rho proteins contribute to cancer progression through their role in regulating cell motility and invasion with an overexpression often seen in tumors. Additionally in cardiovascular diseases Rho affects vascular smooth muscle function influencing blood pressure regulation. In these contexts Rho interacts with proteins such as ROCK and Rac which are also implicated in pathological states. Researchers use anti-Rho and anti-Rho antibodies to study these interactions and understand Rho-related pathologies better.