Abcam, ab239919, Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Thiophosphate ester antibody. Carrier free. Suitable for WB and reacts with Chemical samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:51-8,
Isotype:IgG,
Carrier free:Yes,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific to thiophosphorylated amino acids.

Product details:
ab239919 is the carrier-free version of
ab92570
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco. It is possible to use
ab92570
ab138911
as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007). After the kinase of interest has accepted ATP-γ-S (
ab138911
), p -Nitrobenzyl mesylate (
ab138910
) can be used to alkylate the thiophospholyation site on the substrates.
ab92570
is introduced to identify the tagged substrates.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The thiophosphate ester often referred to as ATP gamma S is a structural analog of adenosine triphosphate (ATP) where the oxygen is substituted with sulfur at the gamma position. This modification results in resistance to hydrolysis making it useful for studying ATP-dependent processes. Although the exact mass of thiophosphate ester varies depending on the specific derivative it is widely utilized in various cellular and organ systems due to its involvement as an ATP analog. The compound is not expressible in living organisms but it is valuable in experimental designs in laboratory settings.
Biological function summary
The role of the thiophosphate ester centers around its use as a tool for modulating enzymatic activity in cellular processes. It often functions as part of complex biochemical assays to investigate phosphorylation mechanisms. As a gamma phosphate analog it non-hydrolyzably activates kinases and other ATP-binding proteins providing insights into their dynamics and interactions within cellular structures. Researchers commonly use it in studies involving protein phosphorylation and signal transduction.
Pathways
The thiophosphate ester plays a significant role in ATP-dependent phosphorylation pathways particularly those involving protein kinases. Its analog can mimic ATP in signal transduction pathways which leads to sustained activation of pathways like MAPK/ERK important for cell proliferation and differentiation. It shares relations with various kinases such as PKA and PKC which regulate diverse cellular responses. This serves to illustrate how its presence aids in understanding the regulation and execution of these pathways by inhibiting or prolonging their activity.
Thiophosphate ester analogs help researchers examine aberrant kinase signaling linked to cancer and neurodegenerative diseases. By stabilizing interactions with proteins such as p53 in cancer pathways it is possible to evaluate the role of faulty phosphorylation in tumorigenesis. In the context of neurodegenerative disorders the ester assists in investigating abnormal signal transduction pathways that may lead to conditions like Alzheimer’s disease. By providing a platform for studying protein interactions it contributes to the understanding of how improper regulation can lead to disease development and progression.