Abcam, ab239932, Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LIM Kinase 1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR912,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239932 is the carrier-free version of
ab108507
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIM Kinase 1 also known as LIMK1 is a serine/threonine protein kinase that plays a critical role in regulating actin cytoskeleton dynamics. Weighing approximately 72 kDa LIMK1 is expressed in various tissues with significant levels observed in the brain and spinal cord. Some studies highlight its elevated presence in neurons. The LIMK1 gene encodes the protein and its activity is often regulated through phosphorylation processes. Researchers often refer to related proteins like LIMK1/proteina in discussions about similar functions.
Biological function summary
This kinase modulates cell shape migration and differentiation by influencing actin filament networks. LIMK1 associates with regulatory complexes and interacts with several proteins to facilitate cytoskeletal reorganization. It plays an essential part in neural development and synaptic plasticity affecting learning and memory. By phosphorylating key actin-binding proteins like cofilin LIMK1 alters actin dynamics essential for various cellular functions.
Pathways
LIM Kinase 1 influences the RhoA/ROCK and Rac1/Pak pathways both critical for cytoskeletal rearrangements. Within these pathways LIMK1 interacts closely with proteins such as cofilin and is influenced by upstream activators like Rho GTPases. These pathways link it to a broader network of signaling that controls cell movement and stability important for cellular responses in various environmental contexts.
LIMK1 is linked to neurological conditions like Williams-Beuren syndrome and some forms of intellectual disability. Changes in LIMK1 expression or activity can disrupt normal brain function leading to cognitive deficits. Its connection to cofilin and other cytoskeleton-associated proteins plays a role in the pathological development of these conditions underlining its importance in maintaining normal neuronal architecture and connectivity.