Abcam, ab239977, Anti-Epac1 antibody [EPR1672] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Epac1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1672,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239977 is the carrier-free version of
ab109415
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Epac1 also known as Exchange Protein directly Activated by cAMP 1 plays an important role in cellular signaling. It is a guanine nucleotide exchange factor with a molecular mass of about 105 kDa. This protein activates Rap1 and Rap2 small GTPases by promoting the exchange of GDP for GTP. Epac1 is widely expressed in various tissues including the heart brain and immune cells. Its distribution indicates its involvement in multiple physiological processes.
Biological function summary
Epac1 influences cell adhesion proliferation and differentiation. It does not function as a single unit; instead it interacts with other proteins to regulate these cellular processes. Its activity is closely linked with the cAMP signaling pathway where it serves as a mediator of cAMP effects independent of protein kinase A (PKA). The interplay between Epac1 and its associated complexes is essential for proper cellular responses to external stimuli.
Pathways
Epac1 contributes to the cAMP signaling and Ras-related pathways. It functions alongside proteins like Rap1 to relay signals that affect cell adhesion and migration. Within these pathways Epac1 acts as a critical mediator between external signals and intracellular responses. Its interactions with other signaling molecules underline its importance in maintaining cellular functions and responses.
Epac1 has associations with cardiovascular diseases and certain cancers. Its dysregulation can influence heart function linking it with proteins such as PKA and Rap1 in pathological conditions. In cancer altered Epac1 expression may influence tumor growth and metastasis reflecting its involvement with cellular proliferation and migration pathways. Understanding Epac1's role in these conditions can provide insights into potential therapeutic strategies.