Abcam, ab240140, Anti-GNAI2 antibody [EPR9469] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GNAI2 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9469,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240140 is the carrier-free version of
ab157204
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNAI2 also known as Gi2 alpha-subunit is a G protein subunit encoded by the GNAI2 gene. It has a molecular weight of approximately 40 kDa. It influences intracellular signaling pathways by inhibiting adenylyl cyclase and decreasing the levels of cyclic AMP (cAMP) within the cell. GNAI2 is found in a variety of tissues with high expression in the brain heart and lymphoid tissues. This wide expression pattern shows its importance in different physiological processes across various organs.
Biological function summary
GNAI2 functions as part of heterotrimeric G protein complexes. It couples with receptors on the cell surface transmitting signals from activated receptors to downstream effectors. GNAI2 binds with GDP in its inactive state and exchanges it for GTP upon receptor activation which causes the dissociation of the G protein into G alpha and G beta-gamma subunits continuing the signaling cascade. GNAI2 contributes to the modulation of processes such as neurotransmission immune responses and cell proliferation.
Pathways
GNAI2 plays an important role in the MAPK/ERK pathway and the PI3K/Akt signaling pathway. It interacts with various receptors and proteins to regulate cellular responses to external stimuli. For instance through the MAPK/ERK pathway GNAI2 influences gene expression and cell division while in the PI3K/Akt pathway it plays a role in cell survival and apoptosis. Its interaction with proteins like G-beta and G-gamma subunits emphasizes its integrative function in coordinating signal transduction.
GNAI2 has links to certain types of cancer and autoimmune diseases. Mutations or dysregulations in GNAI2 expression can influence cancer progression through abnormal cell signaling pathways. Additionally in autoimmune disorders aberrations in GNAI2 signaling can disrupt immune responses contributing to the pathology of diseases like systemic lupus erythematosus. GNAI2 interacts with proteins like adenylyl cyclase and specific receptor proteins which are important for maintaining normal cellular signaling and their dysregulation can lead to disease.