Abcam, ab240142, Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody. Carrier free. Suitable for IP, ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9471,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

Product details:
ab240142 is the carrier-free version of
ab157455
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eEF1A1 also known as EF-Tu or eEF1A1-L3 is an elongation factor involved in protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the A-site of the ribosome during translation. eEF1A1 exhibits a mass of approximately 50 kDa and is widely expressed in various tissues including the brain heart and skeletal muscle. Its expression level varies indicating that it plays a consistent role in translation machinery but may have tissue-specific functions.
Biological function summary
This elongation factor is important in promoting the accuracy and efficiency of protein translation. It interacts with several components of the protein synthesis machinery and forms part of a larger translational complex. eEF1A1 is involved in ensuring the correct amino acids are added during protein synthesis which affects protein structure and function. In addition to its canonical role it also engages in processes such as actin filament binding nuclear transport and other cell maintenance tasks.
Pathways
The activities of eEF1A1 intersect with key processes involved in cellular growth and proliferation. It plays a significant role in the mTOR signaling pathway which regulates cell growth by controlling protein synthesis. eEF1A1 also interacts with proteins like Rheb an important player in mTOR signaling. By participating in these pathways eEF1A1 links translation with broader regulatory networks that control cell metabolism.
EEF1A1 has connections to cancer and neurodegenerative diseases. Its elevated expression is often observed in several cancer types suggesting a role in tumorigenesis. Additionally misregulation of eEF1A1 may contribute to neurodegenerative disorders such as Alzheimer's disease where alterations in protein synthesis pathways are common. The protein's interaction with tau known in neuropathological conditions highlights its impact on neurodegeneration.