Abcam, ab240211, Anti-STAT5b antibody [EPR16671] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal STAT5b antibody. Carrier free. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16671,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ChIP, IHC-P, WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody shows no cross reactivity with STAT5a.

Product details:
ab240211 is the carrier-free version of
ab178941
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.
Biological function summary
STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.
Pathways
STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.
STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.