Abcam, ab240258, Anti-ENT1 antibody [SP120] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ENT1 antibody. Carrier free. Suitable for ICC, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP120,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240258 is the carrier-free version of
ab182023
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENT1 also known as Equilibrative Nucleoside Transporter 1 or SLC29A1 is a membrane protein responsible for transporting nucleosides across cell membranes. This protein weighs approximately 55 kDa and is expressed in various tissues including the brain heart and liver. The transport activity of ENT1 plays a fundamental role in maintaining cellular nucleoside levels. The efficient transport mechanisms of ENT1 highlight its significance in regulating the availability of substrates for nucleic acid synthesis.
Biological function summary
This transporter facilitates the uptake and recycling of nucleosides which are essential building blocks for DNA and RNA synthesis. ENT1 mediates the transport of adenosine an important nucleoside involved in multiple cellular functions. It operates as a component of a broader transport system that includes similar proteins like ENT2. This action contributes to cellular processes by controlling nucleoside concentrations impacting cell proliferation and metabolic activities.
Pathways
ENT1 plays a role in the purine salvage pathway and adenosine signaling. It regulates extracellular adenosine levels influencing adenosine receptor-mediated pathways that impact many physiological functions. ENT1 coordinates with proteins such as adenosine kinases to manage the intracellular balance of adenosine therefore integrating into both metabolic and signaling routes. Its role in these pathways affects energy metabolism and supports cellular responses to stress.
ENT1 has been associated with conditions such as cardiovascular diseases and neurological disorders. Altered ENT1 activity can affect adenosine levels leading to variations in blood flow and neurotransmission. In cardiovascular diseases ENT1 modulates adenosine's cardioprotective effects. In neurological contexts dysfunction of ENT1 relates to disorders involving adenosine receptor signaling where its interaction with proteins like ADA (adenosine deaminase) can influence disease progression.