Abcam, ab240318, Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Nkx2.2 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14638,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240318 is the carrier-free version of
ab191077
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nkx2.2 also known as NK2 homeobox 2 is a transcription factor weighing approximately 29 kDa. It plays a pivotal role in early neural development and is important for the differentiation of oligodendrocytes in the central nervous system. Nkx2.2 is expressed in the developing brain and spinal cord. Researchers often use Nkx2.2 immunohistochemistry (IHC) to study its spatial and temporal expression patterns during embryonic development. This protein is part of a group that includes other members such as Nkx2-1 and Nkx2-5 and it shares conserved domains with them.
Biological function summary
Nkx2.2 influences the regulation of specific genes essential for cell fate determination. It interacts with other factors in a regulatory network forming complexes that modulate gene expression necessary for the maturation of neural precursor cells. Notably Nkx2.2 is required for the development of insulin-producing pancreatic beta cells where it works in conjunction with other transcription factors like Pdx1 and Foxa2. Its ability to bind DNA and promote specific gene transcription positions it as an important regulator in the networks driving neural and pancreatic tissue specification.
Pathways
Nkx2.2 is important in the Sonic Hedgehog (Shh) signaling pathway which is instrumental for embryonic neural tube patterning. The Shh pathway directs Nkx2.2 expression in response to morphogen gradients to determine distinct neural progenitor domains. Nkx2.2 expression is closely linked with the transcription factor Olig2 which aligns with specific domains in the ventral neural tube. Moreover Nkx2.2 is involved in the Wnt signaling pathway playing a role in the coordination of cell differentiation and proliferation.
Nkx2.2 has associations with glioma and type 1 diabetes. Abnormal expression may contribute to glioma development by disrupting normal oligodendrocyte precursor cell development. Nkx2.2 dysregulation can also interfere with insulin regulation implicating it in type 1 diabetes progression especially through its interaction with Pdx1 an important player in pancreas development. Understanding Nkx2.2's role in these conditions aids in deciphering underlying mechanisms and provides potential therapeutic targets.