Abcam, ab240385, Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MASH1/Achaete-scute homolog 1 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, ChIP-seq and reacts with Rat, Human, Mouse samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19840,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human, Rat,
Applications:WB, IHC-P, ICC/IF, ChIP-seq, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The fluorescence IHC images were provided by a collaborator. Our in-house testing failed in fluorescence IHC. We do not guarantee fluorescence IHC.

Product details:
ab240385 is the carrier-free version of
ab211327
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.
Biological function summary
This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.
Pathways
MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.
MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.