Abcam, ab240596, Anti-DENTT antibody

Size: 100µg
Rabbit Polyclonal DENTT antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TSPYL2 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TSPYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9H2G4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DENTT also known as DEP domain-containing protein 5 (DEPDC5) is a member of the GATOR complex. It has a molecular mass of approximately 160 kDa. DENTT is expressed in various human tissues with high levels in the brain and skeletal muscle. This protein plays a role in the mechanisms of nutrient sensing and detecting energy levels within cells.
Biological function summary
DENTT regulates the activity of the mechanistic target of rapamycin complex 1 (mTORC1). As part of the GATOR1 complex it serves to inhibit mTORC1 in response to nutrient levels. By doing so DENTT participates in cellular processes such as growth and metabolism. Its activity is linked to the control of cell size and autophagy.
Pathways
DENTT is involved in the mTOR signaling pathway an important regulator of cell growth and proliferation. It interacts with proteins such as NPRL2 and NPRL3 which form part of the same GATOR1 complex. The interplay between DENTT and these proteins modulates mTORC1 activity aligning cellular metabolism with nutrient availability.
Alterations in DENTT are implicated in familial focal epilepsies. Mutations within the DEPDC5 gene can disturb mTOR signaling leading to imbalanced neuronal excitability. Additionally aberrant DENTT activity connects with proteins like RHEB known to drive mTORC1 activity which contributes to the pathogenesis of certain epileptic conditions.