Abcam, ab24093, Anti-HL60 antibody [IPO-M6]

Size: 100µg
Mouse Monoclonal HL-60 antibody. Suitable for IHC-Fr, Flow Cyt, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing HL60 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:IPO-M6,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-Fr, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Reacts with human leukemia cell line HL60 and immuno-precipitates with two proteins with MW of 48 and 52 kDa. ab24093 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.

Product details:
This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HL60 cell line named for its original derivation from human promyelocytic leukemia serves as a model for studying various aspects of blood cell biology and cancer research. HL60 cells are suspension cells and feature the unique ability to differentiate into several cell types such as granulocytes and macrophage-like cells depending on the stimuli. The cells express markers for early hematopoietic progenitors as well as proteins relevant to immune response. Mass-specific information for the HL60 line is not applicable as it refers to a cell line rather than a solitary protein or molecule.
Biological function summary
HL60 cells provide insights into modulations within the hematopoietic lineage. Scientists frequently use it to study the process of myeloid differentiation offering a stable platform for experiments involving gene expression and genetic modifications. The cell line is not part of a specific protein complex but plays an essential role when studying signal transduction pathways affecting myeloid cell differentiation. Researchers widely apply these cells to analyze transcription factors and cytokine interactions.
Pathways
The HL60 cell line facilitates exploration of the mitogen-activated protein kinase (MAPK) pathway and the role it plays in cell proliferation and differentiation. This pathway interacts with key proteins such as extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) influencing cellular outcomes by receiving and interpreting a multitude of metabolic and external signals. HL60 serves as a powerful tool for dissecting molecular regulation and signal integration within these pathways.
The HL60 cell line contributes significantly to the understanding of acute promyelocytic leukemia given its original derivation from this cancer type. Researchers employ these cells to test therapeutic interventions and understand drug resistance mechanisms related to leukemia. Moreover leading-edge studies utilize HL60 cells to explore the effects of new drugs targeting the modifications of the proteins involved in the disease such as PML-RARα the oncogenic driver in promyelocytic leukemia.