Abcam, ab240975, Anti-DOHH antibody [EPR16730] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DOHH antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16730,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240975 is the carrier-free version of
ab197587
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The deoxyhypusine hydroxylase (DOHH) enzyme also known as hDOHH catalyzes the final step in the post-translational synthesis of the unusual amino acid hypusine. DOHH modifies deoxyhypusine into hypusine by hydroxylation a reaction critical in activating the eukaryotic initiation factor 5A (eIF5A). The human DOHH protein has a mass of approximately 36 kDa and is ubiquitously expressed in various tissues reflecting its general significance in protein processing.
Biological function summary
Proteins such as DOHH play important roles in cellular growth and proliferation. DOHH functions by converting inactive eIF5A precursors into their active form which can influence mRNA translation. This enzyme does not form part of a larger complex with other proteins but its activity is essential for the hypusine modification necessary to activate eIF5A directly influencing protein synthesis.
Pathways
DOHH operates in the hypusine biosynthesis pathway which is important for cell proliferation and differentiation. DOHH acts after the enzyme deoxyhypusine synthase (DHS) introduces a deoxyhypusine residue into eIF5A illustrating the close relationship between these two proteins in this cascade. The activation of eIF5A through hypusination impacts diverse pathways that regulate cell cycle progression and apoptosis.
DOHH activity has implications in cancer and neurodegenerative diseases. Altered expression or malfunction of DOHH can influence eIF5A activity affecting cell growth and survival and therefore contributing to oncogenesis. In neurodegenerative conditions abnormal hypusine synthesis may disrupt neuronal integrity and function. The linked protein eIF5A plays a role in these diseases demonstrating the broader impact of DOHH on health outcomes.