Abcam, ab240979, Anti-Optineurin antibody [EPR20654] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal Optineurin antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20654,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, IHC-Fr, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240979 is the carrier-free version of
ab213556
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Optineurin also known as OPTN is a multifunctional protein of approximately 67 kDa. It is expressed in various tissues including the retina where it plays a role in maintaining cellular functions. Optineurin interacts with several binding partners to execute its roles within the cell. It is involved in intracellular trafficking signaling and inflammation processes. As an essential component of the cellular machinery optineurin aids in proper cellular communication and responses.
Biological function summary
Optineurin influences different cellular processes notably autophagy and regulation of inflammation. It participates in forming a complex with other proteins to facilitate cargo transport and degradation via the autophagy-lysosome pathway. This function is important for removing damaged proteins and organelles ensuring cellular homeostasis. Inflammation regulation occurs through optineurin's ability to inhibit certain signaling pathways therefore modulating immune responses and preventing excessive inflammation.
Pathways
Optineurin plays a role in the NF-kB signaling pathway and the autophagic process. In NF-kB signaling it acts as a negative regulator by degrading specific signaling molecules. This regulation is necessary to prevent chronic inflammation and maintain immune balance. Within autophagy optineurin collaborates with other autophagy-related proteins like LC3 to facilitate the sequestration and clearance of cellular components preventing accumulation of damaged cellular materials.
Optineurin connects to conditions like glaucoma and amyotrophic lateral sclerosis (ALS). Mutations in the optineurin gene link to glaucoma as they can impair autophagy and lead to optic nerve damage. In the context of ALS optineurin mutations disrupt normal cellular functions contributing to motor neuron degeneration. The protein TDP-43 is often associated with ALS and interacts with optineurin in pathological processes affecting disease progression.