Abcam, ab241278, Anti-WIPI2 antibody

Size: 100µg
Rabbit Polyclonal WIPI2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WIPI2 aa 400 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human WIPI2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y4P8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to WIPI2 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WIPI2 also known as WD repeat domain phosphoinositide-interacting protein 2 functions as a critical component in autophagy a cellular degradation process. This protein weighs approximately 46 kDa and plays an important role in the initiation of autophagosome formation by binding to phosphatidylinositol 3-phosphate on lipid bilayers. Its expression is detected in various tissues with significant abundance in skeletal muscle heart and brain. These expression patterns suggest a broad involvement in cellular regulation and maintenance.
Biological function summary
WIPI2 serves as an important scaffold in the formation of the autophagy machinery. It is part of a larger complex that includes key proteins like Atg16L1 and ULK1 which are essential in the autophagy process. WIPI2 assists in recruiting additional autophagic factors to the developing autophagosome contributing to the membrane curvature and expansion necessary for cargo sequestration. Its activity ensures the efficient turnover of damaged organelles and proteins maintaining cellular health and homeostasis.
Pathways
WIPI2 integrates into the autophagy and PI3K/Akt signaling pathways. Each of these pathways plays a significant role in cellular response to nutrient deprivation and stress. In the autophagy pathway WIPI2 directly interacts with proteins like LC3 facilitating the biogenesis of autophagosomes. Through the PI3K/Akt pathway WIPI2 supports cell survival and growth regulation linking metabolic status with autophagic responses. This interaction highlights its importance in cellular adaptation to environmental changes.
WIPI2 shows a strong connection to neurodegenerative diseases and cancer. Dysfunction in WIPI2's activity has been implicated in the progression of neurodegenerative disorders like Parkinson's Disease where impaired autophagy contributes to the accumulation of misfolded proteins. In cancer altered WIPI2 expression and function are observed linking it with tumorigenesis and cancer cell survival potentially through interactions with proteins such as mTOR and AMPK. Understanding WIPI2’s role in these conditions could lead to novel therapeutic strategies that target autophagy modulation.