Abcam, ab241394, Anti-GAL4 antibody [EPR12710(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GAL4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12710(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab241394 is the carrier-free version of
ab175185
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GAL4 also known as a transcription factor is an important protein in yeast. It plays a critical role in regulating the transcription of genes involved in galactose metabolism. The GAL4 protein has a mass of approximately 881 amino acids and is mainly expressed in the nucleus of yeast cells. Common parts of GAL4 include the GAL4 DBD (DNA-binding domain) and GAL4 AD (activation domain) which are critical for its function. Although similar sounding GAL4 should not be confused with galectin-4 or galectin 4 which are different proteins.
Biological function summary
The GAL4 transcription factor functions as a major regulator of galactose-responsive genes. It acts as part of a complex binding with its DNA target sites to initiate transcription of GAL genes like GAL1 GAL7 and GAL10. GAL4 protein recruits transcriptional machinery through its activation domain working alongside other factors to turn on specific gene expression in response to the presence of galactose. This capacity for gene regulation underlines its essential role in sugar metabolism in yeast.
Pathways
GAL4 is intimately involved in the GAL gene regulatory pathway that manages the cellular response to galactose. In this pathway proteins such as GAL80 inhibit GAL4 function under non-inducing conditions. However in the presence of galactose GAL3 binds and neutralizes GAL80 liberating GAL4 to activate gene expression. This system exemplifies a dynamic interaction in yeast allowing precise gene regulation in accordance with sugar availability.
While GAL4 is specifically a yeast protein and does not directly relate to human diseases its functional analogs and concepts have implications in genetic research and synthetic biology. For example GAL4 system is widely used in Drosophila and other model organisms to study gene expression patterns and related disorders. This system compared with mammalian transcription factors provides a foundational methodology for exploring genetic regulation mechanisms and potential implications in understanding metabolic disorders.