Abcam, ab241413, Anti-HECTD3 antibody

Size: 100µg
Rabbit Polyclonal HECTD3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q5T447

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab241413 was affinity purified using an epitope specific to HECTD3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HECTD3 also known as hectd3 ubiquitin protein ligase acts as an E3 ubiquitin ligase. It plays an important role in tagging proteins for degradation through the ubiquitin-proteasome system. The molecular weight of HECTD3 is approximately 110 kDa. HECTD3 is mainly expressed in the brain and various immune cells indicating its involvement in neurological and immune functions.
Biological function summary
The enzyme facilitates the regulation of proteins by adding ubiquitin molecules which marks them for degradation or signaling. This function helps control the levels of target proteins and maintain cellular homeostasis. HECTD3 often forms part of a protein complex enabling its ligase activity to be moderated by interacting proteins.
Pathways
The enzyme integrates into the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome system HECTD3 collaborates with other E2 and E1 enzymes to achieve efficient ubiquitination. It interacts directly with IκB proteins in the NF-kB signaling pathway impacting inflammatory and immune responses by controlling the stability and activity of these proteins.
HECTD3's malfunctions correlate to various cancers and immune disorders. For example its dysregulation affects certain types of leukemia where it modifies the degradation process of tumor suppressor proteins. Moreover HECTD3 interacts with proteins such as TP53 which also plays a significant role in the development of cancers. Changes in HECTD3 expression in immune cells influence immune disorders connecting it to autoimmune diseases where immune regulation becomes disrupted.