Abcam, ab24186, Anti-CARD8 antibody

Size: 100µg
Anti-CARD8 antibody (ab24186) is a rabbit polyclonal antibody detecting CARD8 in Western Blot, ICC/IF . Suitable for Human . - Over 10 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CARD8. The exact immunogen used to generate this antibody is proprietary information.Q9Y2G2,
Specificity:ab24186 reacts with CARD8 (CARDINAL/TUCAN) protein.

Product details:
What is this antibody validated in?
Anti-CARD8 antibody (ab24186) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of CARD8?
Anti-CARD8 (ab24186) specifically detects a band for CARD8 (UniProt: Q9Y2G2) at a molecular weight of 49kDa.
Trusted by the scientific community
Anti-CARD8 (ab24186) was first used in a scientific publication in 2005 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CARD8 also known as TUCAN or CARDINAL is a member of the CED-4 family of apoptosis proteins. This protein has a molecular mass of approximately 48 kDa. CARD8 expresses mostly in immune cells like monocytes and lymphocytes. Mechanically it possesses a caspase recruitment domain (CARD) which engages in protein-protein interactions. CARD8 interacts with components of the inflammasome playing a role in regulating apoptosis and inflammatory responses.
Biological function summary
CARD8 serves as a regulatory component in the innate immune system. It belongs to the NLR family and forms part of the inflammasome complex. Inflammasomes are essential for activating pro-inflammatory cytokines like IL-1Β. CARD8 modulates the activity of caspase-1 controlling cytokine maturation and secretion. By inhibiting the activation of these pro-inflammatory cytokines CARD8 affects the immune response during infections and inflammatory conditions.
Pathways
CARD8 is involved in both the inflammasome and apoptosis pathways. In the inflammasome pathway CARD8 interacts with proteins like NLRP3 and ASC to regulate caspase-1 activation. In the apoptosis pathway it connects with other proteins like caspase-9 influencing cell death processes. The integration into these pathways highlights CARD8's role in balancing immune response and cell survival.
CARD8 has links to various inflammatory diseases and cancers. CARD8 impacts ailments such as rheumatoid arthritis and certain types of cancer by its ability to influence apoptosis and inflammation. In rheumatoid arthritis altered CARD8 function can lead to excessive inflammation partly through its connection with NLRP3 and caspase-1. In cancer its dysregulation impacts tumor survival pathways potentially linking it with other proteins like caspase-9.