Abcam, ab242052, Anti-MEK4/MKK4 antibody [EP615Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MEK4/MKK4 phospho S257 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP615Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab242052 is the carrier-free version of
ab33912
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab33912 is a purified IgG., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEK4 also known as MKK4 is a protein kinase with a molecular weight of approximately 43 kDa. It functions as a dual-specificity kinase that phosphorylates and activates other kinases in cellular signaling pathways. MEK4 appears in various tissues including heart brain and skeletal muscle. It acts as an essential component in transmitting extracellular signals to downstream effectors and is involved in critical cellular processes like proliferation differentiation and apoptosis.
Biological function summary
MEK4 acts as an upstream kinase in the MAPK signaling cascade. Through its role in this pathway it is part of a larger signaling complex. MEK4 specifically activates JUN N-terminal kinase (JNK) by phosphorylation. JNK further regulates gene expression by influencing transcription factors impacting cellular responses. This makes MEK4 a pivotal element in facilitating messenger roles that allow cells to adapt to changing environments such as stress responses and immune reactions.
Pathways
MEK4 plays significant roles in the MAPK and stress-activated protein kinase (SAPK) pathways. In the MAPK pathway MEK4 phosphorylates JNK thereby influencing cellular responses to environmental changes. Within the SAPK pathway MEK4 interacts with other kinases such as MKK7 to modulate cellular responses further. The flow of signals through these pathways affects cell fate cell cycle regulation and programmed cell death demonstrating its comprehensive influence on cell physiology.
MEK4 shows involvement in cancer progression and neurodegenerative diseases. Abnormal activation of MEK4 can contribute to tumor growth and metastasis in cancers such as prostate and ovarian cancer by enhancing JNK signaling. In neurodegenerative disorders deregulation of MEK4-related pathways might lead to neuronal damage or apoptosis. Proteins like JNK and other MAP kinases become dysregulated in these conditions illustrating the interconnected role of MEK4 in disease mechanisms. Phospho flow cytometry can be utilized to measure alterations in MEK4 phosphorylation states aiding in elucidating its role in disease contexts.