Abcam, ab242359, Anti-Laminin alpha 4/LAMA4 antibody [CL3185]

Size: 100µL
Mouse Monoclonal Laminin alpha 4/LAMA4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Laminin subunit alpha-4 aa 650-850.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL3185,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Laminin subunit alpha-4 aa 650-850. The exact immunogen used to generate this antibody is proprietary information.Q16363,
Epitope:Binds to an epitope located within the peptide sequence GALARKSALKTRLSD as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Laminin alpha 4 (LAMA4) also known as laminin-8 is a subunit of the laminin protein family which are essential components of the extracellular matrix. This protein has a molecular weight of approximately 210 kDa. LAMA4 mainly expresses in the vasculature including blood vessels and organs with high metabolic rates like the heart and skeletal muscles. It plays a significant role in maintaining the structural integrity of tissues by forming a network that supports cell adhesion and migration.
Biological function summary
Laminin alpha 4 interacts with other laminin subunits such as beta and gamma chains to form heterotrimeric laminin complexes. These laminins are important for cell differentiation proliferation and tissue repair. LAMA4 influences the basement membrane dynamics contributing to the biomechanical properties of cells. Through its binding to integrins and other cell surface receptors LAMA4 activates intracellular signaling cascades necessary for organogenesis and tissue regeneration.
Pathways
Laminin alpha 4 participates actively in the PI3K/AKT pathway important for cell survival and growth. It also plays a role in the MAPK signaling pathway which is important for cell differentiation and response to stress signals. Through these pathways LAMA4 interacts with various proteins including integrins and dystroglycan modulating essential cellular processes and maintaining cellular homeostasis.
LAMA4's role becomes prominent in cancer progression where altered expression can influence tumor invasion and metastasis. Furthermore it connects to cardiovascular disorders especially in influencing angiogenesis and repair of ischemic tissues. In these contexts LAMA4 relates to proteins like integrin alpha6 and beta1 which facilitate aberrant cellular activities in disease processes. Understanding LAMA4's involvement offers potential pathways for therapeutic intervention in these diseases.