Abcam, ab242465, Anti-Eotaxin antibody [EPR16611-20] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Eotaxin antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16611-20,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab242465 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Eotaxin. The recommended pair for sandwich ELISA is:
Capture: ab242465, Mouse Eotaxin Capture Antibody (unconjugated)
Detector:
ab242716
, Mouse Eotaxin Detector Antibody (unconjugated)
The reference range value is 3.9 - 250 pg/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.
Biological function summary
Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.
Pathways
Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.
Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.