Abcam, ab242731, Anti-MMP10 antibody [EPR17017-34] - BSA and Azide free (Capture/Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MMP10 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17017-34,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab242731 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MMP-10. The recommended pair for sandwich ELISA is:
Capture: ab242731, Mouse MMP-10 Capture Antibody (unconjugated)
Detector:
ab280985
, Mouse MMP-10 Detector Antibody (unconjugated)
The reference range value is 78.13-5000 pg/mL.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MMP10 also known as stromelysin-2 is a member of the matrix metalloproteinase (MMP) family. This protein is involved in the degradation of components of the extracellular matrix. MMP10 weighs around 54 kDa in its native form. It gets expressed in various tissues including the skin lung and reproductive organs and plays a part in processes like tissue remodeling and repair. Unlike other MMPs MMP10 does not usually cleave collagen but breaks down proteins like elastin and gelatin. The Luminex MMP assay is a method used to study this protein's expression and regulation.
Biological function summary
MMP10 impacts the restructuring and turnover of the extracellular matrix in both normal and disease conditions. It does not work alone; it operates synergistically with other MMPs in a complex matrix framework. Within tissues MMP10 modifies the integrity of the extracellular environment affecting cell behaviors such as migration and invasion. It steps in during processes like wound healing and inflammation exemplifying its contribution to cellular repair mechanisms and their regulation.
Pathways
The activity of MMP10 intersects with the MAPK and TGF-beta signaling pathways. MMP10 facilitates these pathways by digesting extracellular matrix barriers which then influences cellular responses. MMP10's function is also linked with other metalloproteinases like MMP1 and MMP3 in the pathways. Its regulation and activation play an important role in maintaining cellular homeostasis and responding to environmental cues.
MMP10 is associated with conditions such as cancer and arthritis. In cancer its role in promoting extracellular matrix breakdown facilitates tumor cell invasion and metastasis. In arthritis MMP10 contributes to joint tissue destruction perpetuating the inflammatory cycle. This protein frequently collaborates with MMP13 in these diseases reflecting its significant part in disease progression and pathology.