Abcam, ab243141, Anti-Proinsulin antibody [3B6-19-1]

Size: 100µg / 1mg
Mouse Recombinant Monoclonal Insulin antibody. Suitable for Dot, IHC-P, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3B6-19-1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, Flow Cyt (Intra), DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Proinsulin is a precursor molecule important for insulin production. Its alternate names include 'INS-IGF2' or 'preproinsulin'. It has a molecular mass of approximately 9 kDa. The primary expression occurs in the beta cells of the pancreatic islets. Proinsulin undergoes enzymatic cleavage to produce insulin and C-peptide executing a significant transformation as it matures into active insulin which plays an important role in glucose metabolism.
Biological function summary
Proinsulin facilitates the synthesis and release of insulin an essential process involving the conversion from proinsulin to insulin. Proinsulin itself does not operate as part of a complex but requires processing enzymes like prohormone convertase to mature into functional insulin. The efficient conversion of proinsulin to insulin allows cells to maintain glucose homeostasis highlighting its importance in cellular energy usage.
Pathways
Insulin biosynthesis and secretion and the glucose homeostasis pathway rely heavily on proinsulin. In the insulin biosynthesis pathway proinsulin acts upstream of insulin the hormone directly regulating blood glucose levels. The pathways involve interaction with proteins such as proprotein convertase subtilisin/kexin type 1 (PCSK1) which participates in the conversion of proinsulin to insulin. Through these pathways proinsulin ensures proper energy regulation and balances the body's glucose supply with cellular demands.
Aberrations in proinsulin processing or levels closely associate with diabetes mellitus and hyperinsulinemia. Diabetes mellitus characterized by insulin deficiency or resistance can result from insufficient proinsulin conversion to insulin. Hyperinsulinemia involves excess levels of insulin-related peptides sometimes due to incomplete proinsulin processing. Disorders affecting proinsulin often involve its relationship with insulin where inadequate or excess production directly influences blood glucose regulation and disease progression.