Abcam, ab243442, Anti-ZSWIM8 antibody

Size: 100µL
Rabbit Polyclonal ZSWIM8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZSWIM8 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZSWIM8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.A7E2V4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZSWIM8 also known as Zinc Finger SWIM-Type Containing 8 is an E3 ubiquitin ligase complex with an approximate mass of 180 kDa. This protein plays a role in the ubiquitination process which systemically adds ubiquitin to substrate proteins to mark them for degradation. ZSWIM8 is expressed in various tissues including the heart liver and skeletal muscle indicating its broad functional involvement within the human body.
Biological function summary
ZSWIM8 participates in the selective degradation of mRNA acting as a modifier within the mRNA decay pathway. It forms a complex with other proteins including ZNF598 and TRIP12 to mediate this process efficiently. By tagging specific RNA for degradation ZSWIM8 helps regulate gene expression and maintain cellular homeostasis. This activity implies its importance in cellular differentiation and response mechanisms under various physiological conditions.
Pathways
ZSWIM8 is significant in the nonsense-mediated decay (NMD) pathway which monitors mRNA for translation errors and prevents the accumulation of potentially harmful or non-functional proteins. This pathway involves cooperation with factors such as UPF1 and SMG5 that enhance the recognition and subsequent decay of flawed RNA transcripts. Through these interactions ZSWIM8 aids in preserving the integrity and accuracy of protein synthesis a critical task in cellular quality control mechanisms.
Defects in ZSWIM8 activity have associations with neurodevelopmental and muscular disorders. Disruption in the mRNA decay process can contribute to conditions such as intellectual disabilities and dystrophic pathologies. Its interaction with proteins like UPF1 and other decay-related factors highlights its potential involvement in these disorders. Studying the function and regulation of ZSWIM8 could offer insights into novel therapeutic strategies for managing these diseases.