Abcam, ab244371, Anti-UCHL3 antibody

Size: 100µL
Rabbit Polyclonal UCHL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ubiquitin carboxyl-terminal hydrolase isozyme L3 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Ubiquitin carboxyl-terminal hydrolase isozyme L3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P15374

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UCHL3 also known as ubiquitin carboxyl-terminal hydrolase L3 is a deubiquitinating enzyme with a molecular mass of approximately 26 kDa. This protein plays a mechanical role in removing ubiquitin from polyubiquitinated proteins therefore controlling their degradation and recycling in the proteasome pathway. UCHL3 expresses widely across tissues with significant presence observed in the brain kidney and testis. It maintains protein homeostasis by ensuring that ubiquitin is available for various cellular processes making it an essential regulator of protein turnover.
Biological function summary
Ubiquitin carboxyl-terminal hydrolase L3 functions to regulate protein stability and signaling pathways by reversing protein ubiquitination. UCHL3 acts independently but can sometimes associate with complexes involved in cellular stress responses. Deubiquitinase activity makes it a critical regulator that modulates proteins involved in DNA repair transcriptional regulation and cell cycle progression. This regulation has vast implications for cellular growth differentiation and survival.
Pathways
UCHL3 plays significant roles in the ubiquitin-proteasome pathway and the DNA damage response pathway. By participating in these pathways UCHL3 influences cellular pathways that control protein quality control and genomic stability. It interacts with proteins such as p53 and BRCA1 influencing their stability and function. UCHL3's activity affects cellular responses to stress and repair processes thereby maintaining cellular integrity and promoting cancer prevention mechanisms.
UCHL3 has connections to neurodegenerative disorders and cancer. The dysregulation of UCHL3 contributes to neurodegenerative diseases like Alzheimer's where protein aggregation occurs due to impaired ubiquitin processing. Its role in cancer includes influencing tumor suppressors and oncogenes through pathways involving proteins like p53 affecting cell cycle regulation and apoptosis. These associations make UCHL3 a relevant target for therapy development in these diseases.