Abcam, ab244399, Anti-RPN2 antibody

Size: 100µL
Rabbit Polyclonal RPN2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 aa 300-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.P04844

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPN2 also known as Ribophorin II is a subunit of the oligosaccharyltransferase (OST) complex. This protein has a mass of approximately 68 kDa. It is expressed mainly in the rough endoplasmic reticulum playing a role in the N-linked glycosylation of nascent polypeptides. This process is essential for protein folding and stability. RPN2 helps transfer oligosaccharide chains to asparagine residues in nascent proteins.
Biological function summary
RPN2 is integral to the OST complex which consists of multiple subunits each contributing to the glycosylation process. By enabling glycosylation RPN2 ensures proper protein folding allowing proteins to achieve functional conformation and maintain stability. This process affects various cellular activities including protein secretion cell surface receptor expression and immune function.
Pathways
RPN2 plays a significant role in the N-linked glycosylation pathway. This pathway involves the attachment of glycans to proteins influencing their folding distribution and function. RPN2 interacts with other proteins such as STT3A and STT3B which are essential components of the OST complex. This interaction modulates protein modification during synthesis and affects downstream pathways like cell signaling and immune response.
RPN2 has connections to cancer and congenital disorders of glycosylation (CDG). Its altered expression in cancer cells correlates with tumor progression and metastasis where it may impact the function of proteins such as EGFR involved in cell growth and survival. In CDG deficiencies in glycosylation due to RPN2 malfunction can lead to a range of symptoms affecting multiple body systems linking it with several glycosylation-related proteins.