Abcam, ab24520, Anti-68kDa Neurofilament/NF-L antibody

Size: 50µL
Chicken Polyclonal 68kDa Neurofilament/NF-L antibody. Suitable for IHC, WB and reacts with Rat, Mouse, Cow samples. Cited in 3 publications.
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Reacts with:Mouse, Rat, Cow,
Applications:IHC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.

Properties and Storage Information:
Form-Liquid, Purification notes-The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation., Storage buffer-Preservative: 0.065% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
Biological function summary
The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.
Pathways
Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.
Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.