Abcam, ab245249, Anti-LINE-1 ORF1p antibody [EPR22227-6]

Size: 20µL / 100µL / 1mL
Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249) is a rabbit monoclonal antibody detecting LINE-1 ORF1p in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22227-6,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of LINE-1 ORF1p?
Anti-LINE-1 ORF1p [EPR22227-6] (ab245249) specifically detects a band for LINE-1 ORF1p (UniProt: Q9UN81) at a molecular weight of 40kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR22227-6] also available for your convenience: ab245249, Carrier free -
ab246317
, Alexa Fluor® 647 -
ab314927
, Alexa Fluor® 488 -
ab315135
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LINE-1 ORF1p also called L1 ORF1p is a protein encoded by the open reading frame 1 (ORF1) of the long interspersed nuclear element-1 (LINE-1 or L1) family. These proteins typically have a mass of around 40 kDa. They are predominantly expressed in the germ line and embryonic tissues but can also be found in various somatic tissues under abnormal conditions. L1 ORF1p plays a critical role in the retrotransposition process by forming ribonucleoprotein complexes that assist LINE-1 RNA in entering the nucleus and integrating into the genome.
Biological function summary
L1 ORF1p facilitates the mobilization of LINE-1 elements within the genome impacting genome stability and variability. This protein forms a trimeric complex that binds LINE-1 RNA protecting it from degradation. By promoting the retrotransposition of LINE-1 elements L1 ORF1p influences genomic dynamics potentially contributing to genetic diversity but also potentially causing insertions that may lead to genomic instability and associated pathologies.
Pathways
The activity of L1 ORF1p intersects with genome defense mechanisms and cellular stress response pathways. Important pathways include the single-stranded DNA break repair system and retrotransposon silencing mechanisms where proteins like TREX1 and MOV10 play related roles. These pathways help control the activity of transposable elements and maintain genomic integrity providing a first line of defense against potential disruptions caused by retrotransposition events.
Aberrant expression or dysregulation of L1 ORF1p has been linked to cancer and neurodegenerative disorders. Elevated levels of L1 ORF1p have been observed in some cancers such as lung and breast cancer where it may contribute to genomic instability. Furthermore Alzheimer's disease has shown L1 activity potentially involving interaction with amyloid precursor protein (APP) and contributing to disease pathology. Understanding these associations highlights the relevance of L1 ORF1p as a potential target for therapeutic intervention in these diseases.