Abcam, ab245332, Anti-TLS/FUS antibody

Size: 100µg
Rabbit Polyclonal TLS/FUS antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FUS aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human FUS aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P35637

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245332 was affinity purified using an epitope specific to TLS/FUS immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TLS/FUS also known as FUS and the FUS protein is an RNA-binding protein involved in various cellular processes. It has a molecular weight of approximately 53 kDa. Expressed extensively in the nucleus FUS/TLS relocates to the cytoplasm under stress conditions. It plays mechanical roles in transcription regulation RNA splicing and mRNA transport. Scientists sometimes refer to FUS as the 'fused in sarcoma' protein because of its involvement in gene fusion events.
Biological function summary
The FUS protein aids in the maintenance and stabilization of mRNA molecules and participates in the formation of stress granules distinct cytoplasmic aggregates of proteins and RNAs. It forms part of a large protein complex alongside other RNA-binding proteins. FUS stabilizes pre-mRNA structures which affects protein expression patterns essential for cell survival and differentiation. Its ability to bind to RNA and DNA indicates its fundamental role in genomic stability.
Pathways
The functions of FUS help regulate RNA metabolism-related pathways and cellular stress response pathways. FUS interacts with proteins like TAF15 and EWSR1 within these pathways showing a complex network of interactions that contribute to RNA maturation and stress granule dynamic formation. The activity of FUS in these pathways ultimately affects cellular homeostasis gene expression modulation and response to cellular stress.
FUS protein mutations or mislocalizations relate to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). These conditions are characterized by the accumulation of FUS or FUS-related aggregates in the cytoplasm leading to neuronal cell death. FUS is also connected to other RNA-binding proteins like TDP-43 which also mislocalizes and forms aggregates in these disorders highlighting the importance of protein homeostasis and normal RNA metabolism in the prevention of these diseases.