Abcam, ab245384, Anti-XPC antibody

Size: 100µg
Rabbit Polyclonal XPC antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human XPC aa 800-900.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human XPC aa 800-900. The exact immunogen used to generate this antibody is proprietary information.Q01831

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245384 was affinity purified using an epitope specific to XPC immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Xeroderma Pigmentosum Complementation Group C (XPC) protein is essential for the nucleotide excision repair (NER) mechanism repairing DNA damage. XPC with a molecular weight of approximately 125 kDa recognises and binds to damaged DNA that has bulky adducts. It is expressed in various human tissues with higher expression levels in proliferating cells and skin. XPC plays a critical role in DNA testing and maintenance making it vital for maintaining genome stability.
Biological function summary
The XPC protein acts as a damage sensor within the NER pathway and functions as part of the XPC-HR23B complex. This complex identifies DNA helix distortions and signals for repair by recruiting other proteins to the damage site. XPC functions by unwinding the DNA and allowing necessary repair enzymes such as DNA helicases and nucleases to perform their functions. This repair process ensures that the DNA is intact and capable of proper transcription and cell replication.
Pathways
XPC operates within the nucleotide excision repair pathway which is essential for removing a wide range of DNA lesions caused by ultraviolet (UV) irradiation and chemical agents. It operates in conjunction with proteins such as XPA RPA and the TFIIH complex to execute DNA repair. XPC is also linked to the global genomic repair sub-pathway of NER where it plays a leading role in identifying DNA damage across the entire genome.
Ineffective XPC function is associated with Xeroderma Pigmentosum (XP) a genetic disorder causing extreme sensitivity to UV light and increased skin cancer risk. Mutations in the XPC gene impede DNA repair efficiency leading to the accumulation of damage. XPC is also connected to skin cancer development as its malfunction can cause failure to repair UV-induced lesions. Other proteins like XPA and XPE also show connections to these disorders highlighting their critical roles in maintaining DNA integrity and preventing disease.