Abcam, ab245529, Anti-ARID2 antibody

Size: 100µg
Rabbit Polyclonal ARID2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARID2 aa 1600-1650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARID2 aa 1600-1650. The exact immunogen used to generate this antibody is proprietary information.Q68CP9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245529 was affinity purified using an epitope specific to ARID2 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARID2 also known as AT-rich interactive domain-containing protein 2 is a component of the SWI/SNF chromatin remodeling complex. It has a molecular mass of approximately 183 kDa. ARID2 is expressed in various tissues with high levels found in the liver kidney and testis. In the cell nucleus it functions as a DNA-binding protein playing a role in modulating chromatin structure through its interaction with other chromatin-associated proteins.
Biological function summary
ARID2 contributes to regulating gene expression by remodeling chromatin allowing transcription factors access to DNA. It acts as a part of the PBAF subtype of the SWI/SNF complex which includes proteins like BRG1 and BAF200. This complex influences transcriptional activation and repression aiding the regulation of genes important for cellular differentiation and development. Loss or mutation of ARID2 affects normal biological processes indicating its role in ensuring proper cellular function.
Pathways
ARID2 plays a role in the RAS signaling and the Hedgehog signaling pathways. It interacts with proteins such as BAF155 and BAF170 which are essential for recruiting and assembling the PBAF complex on chromatin. These pathways are critical for cellular proliferation and differentiation ensuring that cells respond accurately to signals which is vital for maintaining tissue homeostasis and proper organismal development.
ARID2 mutations are linked to hepatocellular carcinoma and malignant melanoma. These mutations are associated with abnormalities in chromatin remodeling that lead to transcriptional dysregulation. The target is also connected to proteins like BRD9 and BRD7 within the context of cancer which are involved in tumor suppression. Alterations in ARID2 might contribute to the progression and aggressiveness of these cancers by disrupting normal chromatin dynamics and gene expression patterns.