Abcam, ab245538, Anti-XPB antibody

Size: 100µg
Rabbit Polyclonal XPB antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERCC3 aa 700 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ERCC3 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P19447

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245538 was affinity purified using an epitope specific to XPB immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XPB also known as ERCC3 is part of the helicase family. It has an approximate mass of 89 kDa. XPB uses ATP to unwind DNA which facilitates various genetic processes. In humans it expresses in tissues where there is active DNA replication and repair occurring including rapidly dividing cells. Its activity is important to the transcription process and DNA damage repair mechanism.
Biological function summary
XPB assists the cellular response to DNA damage by being part of the TFIIH complex which is involved in transcription. This complex plays a role in nucleotide excision repair (NER) where it unwinds DNA segments to allow for lesion repair. In conjunction with other proteins XPB facilitates the resolution of DNA loops and manages the clearance of polymerase during transcription-coupled repair ensuring proper DNA repair and transcription restart.
Pathways
XPB influences both transcription initiation and DNA damage repair being integral to the mechanisms that maintain genome integrity. It interacts closely with proteins like XPD within the NER pathway and its roles in these pathways contribute to the prevention of mutations. XPB's function as a helicase denotes its participation in opening DNA for repair or transcription therefore supporting cell viability and genomic stability.
XPB's deficiency or malfunction links to xeroderma pigmentosum and trichothiodystrophy. These conditions stem from impaired nucleotide excision repair where mutations in XPB affect the efficiency of DNA repair and proper cellular response to damage. Symptoms correlated with these diseases often include increased sensitivity to UV light and neurological deficits connected through pathways involving other TFIIH complex components like XPD signifying the collaboration needed within a cell for effective DNA repair.