Abcam, ab246330, Anti-GRSF1 antibody [EPR16678] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GRSF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16678,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, Flow Cyt (Intra), WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab246330 is the carrier-free version of
ab205531
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The G-rich RNA sequence binding factor 1 known as GRSF1 is a protein involved in RNA processing activities. Alternate names include MGC60760 and GAR1. This protein weighs approximately 48 kDa and demonstrates a wide expression pattern across different tissues with a marked presence in mitochondria. GRSF1 binds specifically to G-rich elements in RNA playing a mechanical role in stabilizing complex RNA structures and facilitating efficient RNA processing.
Biological function summary
The GRSF1 operates as an important player in mitochondrial RNA metabolism. It is known to interact within ribonucleoprotein complexes facilitating the regulation of mitochondrial gene expression. GRSF1 contributes to the maturation and processing of mitochondrial RNA impacting the proper synthesis of components essential for mitochondrial function. It ensures that mitochondrial encoded proteins are synthesized accurately aiding overall cellular energy production.
Pathways
GRSF1 engagement with organellar RNA processing integrates into mitochondrial biogenesis and maintenance pathways. This protein significantly influences the electron transport chain by ensuring the precise maturation of mitochondrial RNAs required for encoding electron transport chain components. It connects with proteins like LRPPRC as part of RNA granules coordinating gene expression regulation to support cellular respiration and energy conversion pathways.
GRSF1 links closely to conditions involving mitochondrial dysfunctions such as mitochondrial myopathy. Alterations in GRSF1 function can result in impaired energy production leading to muscle weakness and other systemic symptoms associated with myopathy. It may also intersect with neurodegenerative disorders where mitochondrial impairment represents a common pathological feature. GRSF1's association with proteins like Twinkle helicase in such diseases emphasizes its broader implications in maintaining mitochondrial integrity and cellular health.