Abcam, ab246831, Nuclear Fast Red Solution

Size: 125mL
Nuclear Fast Red Solution (ab246831) is a ready-to-use counterstain for IHC, ISH and routine histological applications. Suitable for paraffin embedded tissue section, frozen tissue sections and cell smears. - Stains nuclei red and cytoplasm pale pink - Formulation with enhanced stability- no precipitation ensuring a longer shelf-life - Compatible with our Lab essentials imaging workflow offering
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 3 - 5Preservative: 0.05% Proclin 300Constituents: 95% Water, 4.5% Aluminium sulfate, 0.2% Nuclear Fast Red

Product details:
Nuclear Fast Red Solution (ab246831) is a ready-to-use counterstain used in histological applications e.g. IHC and ISH. The reagent has improved stability over current formulations. Current formulations tend to precipitate in cold temperatures such as experienced during winter shipping. In addition, most formulations develop a small amount of precipitate over extended periods of time. This advanced formulation eliminates problems associated with exposure to cold and aging.
Expected results
Nuclei: Red
Cytoplasm: Pale Pink
Control Tissue:
Any well fixed paraffin embedded or frozen tissue section. Cell Smear. Cytospin.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Apply Nuclear Fast Red Solution (ab246831) and incubate for 1-5 minutes.
3. Rinse in 2 changes of distilled water.
4. Dehydrate through graded alcohols.
5. Clear, and mount in synthetic resin.
Other products for staining tissue sections
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient