Abcam, ab246832, Picro-Sirius Red Solution

Size: 500mL
Picro-Sirius Red (PSR) Solution (ab246832) is suitable for histological visualization of muscle and collagen fibers, a major component of the extracellular matrix (ECM) in tissue sections in IHC. Suitable to quantify collagen content, fiber density, and organization. - Used to differentiate collagens type I and type III fibers - Suitable for light microscopy and polarized light microscopy - Proven performance: cited in over 35 publications - Compatible with our Lab essentials imaging workflow offering
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 1 - 3Constituents: 1.2% Picric Acid, 0.1% Direct Red 80

Product details:
Picro-Sirius Red Solution ab246832 is intended for use in the histological visualization of muscle and collagen fibers in tissue sections. The stain may be viewed using standard light microscopy or polarized light resulting in birefringence of collagen fibers. Please refer to
ab150681
Picro Sirius Red Stain Kit (Connective Tissue Stain) for more information on this solution as it is a component of the kit.
Light Microscopy
Collagen: Red
Muscle Fibers: Yellow
Cytoplasm: Yellow
Polarized Light Microscopy
Collagen: Yellow-Orange and Green Birefringence
Control Tissues:
Lung, Uterus, Muscle, Kidney
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Apply adequate Picro-Sirius Red Solution to completely cover tissue section and incubate for 60 minutes.
3. Rinse slide quickly in two changes of Acetic Acid Solution (0.5%).
4. Rinse slide using absolute alcohol.
5. Dehydrate in 2 changes of absolute alcohol, clear, and mount in synthetic resin.
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient