Abcam, ab246904, Anti-Nocturnin antibody

Size: 100µL
Rabbit Polyclonal Nocturnin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NOCT aa 250 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NOCT aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UK39

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Nocturnin protein also known as Ccrn4l is a highly conserved enzyme with a molecular mass of approximately 48 kDa. It functions as a deadenylase involved in the removal of poly(A) tails from mRNA and is active mainly in the cytoplasm. Nocturnin expression occurs widely in tissues with significant levels in the liver adipose tissue and skeletal muscles. Its expression is regulated by the circadian clock indicating that it plays a role in synchronizing metabolic processes with environmental light-dark cycles.
Biological function summary
The functions of Nocturnin extend beyond simple mRNA processing. It influences lipid metabolism and energy homeostasis shaping cellular responses to nutrient intake and fasting. It does not typically form part of large protein complexes but associates with specific mRNA substrates and possibly other RNA-binding proteins. Researchers find its role pivotal in adjusting the cellular metabolism in response to circadian rhythms highlighting its influence on energy baselines and storage mechanisms.
Pathways
Nocturnin interacts with distinct circadian and metabolic pathways. The protein is an important player in the regulation of the circadian rhythm pathway acting in concert with core clock proteins like BMAL1 and CLOCK to modulate metabolic genes. Nocturnin also plays a part in the fatty acid metabolism pathway wherein it aids in coordinating cellular energy states and storage capacities. These interactions highlight its integrative function between timekeeping and nutrient metabolism establishing a link between environmental cues and metabolic responses.
Nocturnin shows associations with metabolic disorders and obesity. Its dysregulation can disrupt normal circadian-regulated metabolic processes leading to an increased risk of metabolic syndrome. This protein may also connect to glucose metabolism disorders through its function within the metabolic pathways potentially influencing insulin sensitivity and glucose homeostasis. The insulin signaling pathway involving proteins like IRS-1 interacts with Nocturnin suggesting that dysregulation in its function could have broader implications in metabolic health.