Abcam, ab247089, Anti-IDH3B antibody

Size: 100µL
Rabbit Polyclonal IDH3B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial aa 50-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial aa 50-200. The exact immunogen used to generate this antibody is proprietary information.O43837

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IDH3B also known as isocitrate dehydrogenase 3 (NAD(+)) beta subunit is an enzyme that plays a critical role in the tricarboxylic acid (TCA) cycle. It catalyzes the conversion of isocitrate to alpha-ketoglutarate coupling this with the reduction of NAD+ to NADH. IDH3B has a molecular weight of approximately 41 kDa. Expression of IDH3B is found predominantly in the mitochondria of most eukaryotic cells including in tissues with high energy demands such as muscle and heart.
Biological function summary
IDH3B functions as a part of the IDH3 enzyme complex which consists of alpha beta and gamma subunits. This complex is essential for regulating the TCA cycle affecting cellular energy metabolism. Through its enzymatic activity IDH3B contributes to maintaining proper levels of NADH which in turn influences ATP production and overall cellular respiration.
Pathways
IDH3B serves a significant role in the TCA cycle and is interconnected with the glutamine metabolism pathway. By converting isocitrate to alpha-ketoglutarate it interfaces with proteins such as IDH3A and IDH3G which are subunits of the same enzyme complex. These interactions ensure a well-regulated flow of metabolites necessary for energy production and reductive biosynthesis.
Mutations or dysregulation of IDH3B have been linked to certain types of cancer and neurological disorders. For example some studies have shown alterations in IDH3B expression in gliomas affecting cellular metabolism and growth. Additionally IDH3B interacts with IDH1 and IDH2 which are other isocitrate dehydrogenases known for their mutated forms in various cancers. Understanding these relationships can provide insights into potential therapeutic avenues.