Abcam, ab247132, Anti-SEPN1/SELN antibody

Size: 100µL
Rabbit Polyclonal SEPN1/SELN antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SELENON aa 450 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SELENON aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9NZV5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SEPN1 also known as SELN or selenoprotein N is a selenoprotein that plays important roles in protecting cells from oxidative stress. It is about 65 kDa in mass and is a member of the selenoprotein family characterized by containing the rare amino acid selenocysteine. Its expression occurs mainly in skeletal muscle though it can be found in various other tissues. SEPN1 primarily localizes in the endoplasmic reticulum where it interacts with other cellular components involved in maintaining redox balance.
Biological function summary
SEPN1 is involved in protecting cells by reducing oxidative stress and maintaining proper calcium homeostasis within the muscular system. It integrates into the molecular chaperone complex influencing the function and stabilization of muscle cells. By modulating calcium dynamics and redox status SEPN1 helps ensure proper muscle contraction and cellular integrity which is essential for normal muscle physiology.
Pathways
The functions of SEPN1 intersect with those involved in redox regulation and calcium signaling. SEPN1 plays a role in the endoplasmic reticulum’s function particularly influencing the oxidative folding of proteins. In this context it works alongside proteins like calreticulin and calnexin important for protein folding and quality control. In calcium signaling SEPN1 affects the pathway by influencing the release and uptake of calcium ions necessary for muscle function and signaling pathways.
SEPN1 links to conditions like SEPN1-related myopathy and muscular dystrophy. SEPN1-related myopathy involves weakness and wasting of muscle tissue often evident from early childhood which affects respiratory and skeletal muscles. The dysfunction of this protein can result in improper calcium regulation and compromised muscle cell protection leading to clinical symptoms. Such disorders sometimes involve mutant forms of the selenoprotein family including selenoprotein T indicating overlapping pathways that contribute to muscle tissue pathology.