Abcam, ab247219, Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Caspase-7 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y33,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.

Product details:
ab247219 is the carrier-free version of
ab32067
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pro Caspase-7 also known as CASP-7 is an important component in the apoptotic machinery functioning as an inactive precursor of the enzyme caspase-7. This zymogen has a molecular mass of approximately 34 kDa and is primarily expressed in the cytosol of cells. It undergoes proteolytic cleavage to become active caspase-7 an important step for executing apoptosis. This conversion allows it to cleave specific substrates leading to cell dismantling. Researchers often refer to it by its other names such as CMH-1 or ICE-LAP3 in scientific literature.
Biological function summary
Pro Caspase-7 is intricately involved in the execution phase of apoptosis. It is a component of a multiprotein complex known as the apoptosome which assembles in response to apoptotic signals. Within this complex pro Caspase-7 undergoes activation and subsequently cleaves various cellular proteins. This action results in the characteristic apoptotic changes like DNA fragmentation and membrane blebbing. Its activity is important in maintaining tissue homeostasis and ensuring the removal of damaged cells.
Pathways
Pro Caspase-7 is deeply integrated into the intrinsic and extrinsic apoptotic pathways. In the intrinsic pathway it operates downstream of mitochondrial signals alongside caspase-3 another executioner caspase. The extrinsic pathway involves the death receptor-mediated signals where caspase-8 can indirectly activate pro Caspase-7. These pathways illustrate interconnectivity often involving cross-activation of caspases guaranteeing efficient cell death regulation.
Dysregulation of pro Caspase-7 activity links to cancer and neurodegenerative diseases. In cancer underactive pro Caspase-7 results in reduced apoptosis aiding in uncontrolled cell proliferation. Conversely overactivation of pro Caspase-7 is implicated in neurodegenerative disorders like Alzheimer's disease due to excessive apoptosis. Its interactions with proteins such as BCL-2 in cancer and Tau in Alzheimer's illustrate its role in disease pathology.