Abcam, ab247374, Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal eIF4EBP1 phospho T45 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2169Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247374 is the carrier-free version of
ab68187
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF4EBP1 eIF4EBP2 and eIF4EBP3 are also known as the eukaryotic translation initiation factor 4E-binding proteins. These proteins regulate translation by binding to eukaryotic translation initiation factor 4E (eIF4E) inhibiting its role in the assembly of the eIF4F complex which is important for cap-dependent translation. eIF4EBP1 weighs approximately 12 kDa eIF4EBP2 around 13 kDa and eIF4EBP3 approximately 13 kDa. These proteins are widely expressed in various tissues with expression levels varying depending on cellular conditions and signaling factors.
Biological function summary
These proteins act as repressors of mRNA translation controlling the initiation phase of protein synthesis. They do so by modulating the availability of eIF4E to form the eIF4F complex when bound preventing the initiation of cap-dependent translation. eIF4EBP proteins are phosphorylated in response to growth factors mitogens and other stimuli which leads to their release from eIF4E allowing translation initiation to proceed. This phosphorylation places eIF4EBP proteins within a complex signaling network that involves mTOR (mechanistic target of rapamycin) signaling.
Pathways
These proteins play an important regulatory role in the mTOR pathway known to control cell growth proliferation and survival. mTOR regulates eIF4EBP phosphorylation status and consequently eIF4E availability impacting protein synthesis rates. In these pathways eIF4EBP proteins interact with proteins like eIF4G which also engage in the translational control machinery. The interplay between these proteins enables cells to respond to nutrient availability and growth signals effectively.
EIF4EBP proteins have links to cancer and metabolic diseases like diabetes. Aberrant mTOR signaling where eIF4EBP proteins are involved can contribute to excessive cell growth and cancer progression as eIF4EBPs regulate translation control. Hyperactive mTOR signaling is also connected to insulin resistance linking eIF4EBP proteins to diabetes. These proteins interact with enzymes like AKT which play roles in these disease processes by further influencing signaling pathways that govern cell metabolism and survival.