Abcam, ab247419, Anti-MCM5 antibody [EP2683Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MCM5 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2683Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), IHC-P, IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247419 is the carrier-free version of
ab75975
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.
Biological function summary
As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.
Pathways
MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.
MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.