Abcam, ab247420, Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HtrA2 / Omi antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247420 is the carrier-free version of
ab75982
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HtrA2 also known as Omi or the Omi protein is a serine protease with a molecular mass of approximately 50 kDa. It is a mitochondrial protein and commonly found in the cytosol following cellular stress. HtrA2/Omi mediates its function through its protease and pro-apoptotic activities. It is involved in the degradation of damaged proteins and exerts an important role in maintaining protein quality control within mitochondria. Researchers often study HtrA2/Omi for its function in apoptosis and Parkinson's disease-related pathways.
Biological function summary
The degradation and signaling processes of HtrA2/Omi protect against proteotoxic stress. It operates within the mitochondria and under stress conditions relocates to the cytosol where it can influence apoptosis. HtrA2/Omi can interact with inhibitors of apoptosis proteins (IAPs) and by doing this promotes apoptosis when necessary. This regulation of cell death links HtrA2/Omi to the cellular quality control mechanisms directly impacting cell survival and health.
Pathways
HtrA2/Omi integrates into the mitochondrial apoptosis pathway and is important in Parkinson's disease pathways. Through its interactions with apoptosis regulators like XIAP HtrA2/Omi facilitates the removal of unwanted cells. Additionally it participates in signaling pathways involving caspases and other pro-apoptotic factors. Its activity ensures proper communication and response within these pathways to maintain cellular balance and respond to stressors or damage.
HtrA2/Omi links to neurodegenerative conditions such as Parkinson's disease and Huntington's disease. Mutations or alterations in expression levels can contribute to these conditions by disrupting normal apoptotic signaling. Other proteins like PINK1 and Parkin are closely related to HtrA2/Omi in the context of Parkinson's disease where they collectively regulate mitochondrial function and health. In such disorders the malfunction of HtrA2/Omi or its interactions leads to impaired cell survival and increased susceptibility to neuronal damage.