Abcam, ab247444, Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RNA polymerase II RPB1 antibody. Carrier free. Suitable for IP, ChIP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1509Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ChIP, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247444 is the carrier-free version of
ab76123
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase II RPB1 also known as POLR2A or polymerase II is a protein that functions as a catalytic core in the transcription machinery. It plays an important role in transcribing DNA into mRNA essential for initiating protein production. RPB1 displays a molecular mass of approximately 220 kDa. It is ubiquitously expressed in eukaryotic cells signifying its extensive involvement across the cellular milieu.
Biological function summary
RNA polymerase II RPB1 orchestrates the assembly of the preinitiation complex collaborating with various transcription factors to enable accurate transcription initiation. It forms an important part of the RNA polymerase II complex which consists of twelve subunits in eukaryotes. This assembly facilitates the synthesis of pre-mRNA and snRNA which are paramount for proper gene expression and RNA processing.
Pathways
RNA polymerase II RPB1 integrates predominantly into the gene expression and regulation pathways including the transcription initiation and elongation processes. It operates alongside transcription factors like TFIIB and TFIIF which assist in positioning RPB1 correctly on the DNA template. It is also associated with signaling pathways such as MAPK which modulates cellular responses including growth and stress.
RNA polymerase II RPB1 has been implicated in conditions like Cockayne syndrome and cancer. Disruptions in its transcriptional functions can lead to defects in DNA repair and cell cycle control contributing to cancer progression. Links with proteins such as p53 a tumor suppressor involved in cell cycle regulation highlight its role in cancer-related pathways. Cockayne syndrome on the other hand arises due to impaired DNA damage response with defects in RNA polymerase II-linked transcription coupling repair.