Abcam, ab247490, Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PIAS1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2581Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247490 is the carrier-free version of
ab77231
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIAS1 and PIAS2 are known as Protein Inhibitors of Activated STAT. PIAS1 has a mass of approximately 72 kDa while PIAS2 is around 66 kDa. These proteins are present in the nucleus and they regulate various transcription factors. They achieve this by attaching SUMO (Small Ubiquitin-like Modifier) proteins to specific substrates a process called SUMOylation. Both PIAS1 and PIAS2 express in various tissues but expression levels can vary with cell type and physiological conditions.
Biological function summary
PIAS1 and PIAS2 proteins play roles in the regulation of gene transcription. They can form complexes with several transcription factors and alter their activity by SUMOylating them. This modification is important because it changes how these transcription factors interact with DNA and other proteins. PIAS proteins sometimes act as co-regulators by either enhancing or repressing transcription factor function. This dual ability influences cellular processes like cytokine signaling and the immune response.
Pathways
PIAS1 and PIAS2 participate in the JAK-STAT signaling pathway which is critical for transmitting signals from cytokines to the cell nucleus. It involves the activation of STAT family proteins such as STAT1 and STAT3. PIAS proteins add a layer of regulation influencing cell growth and differentiation. Another pathway connected with these proteins is the p53 pathway where PIAS1 can interact with p53 to regulate apoptosis and cell cycle arrest. These interactions indicate the versatile roles of PIAS proteins in cellular homeostasis.
PIAS1 and PIAS2 have links to cancer and autoimmune diseases. For example abnormal sumoylation mediated by these proteins can contribute to oncogenesis by disrupting normal cell cycle control involving key players like p53. In autoimmune conditions PIAS proteins may alter immune response through changes in inflammation-related genes. Knowing the interaction of PIAS proteins with transcription factors such as STAT1 helps to understand the complex gene regulation changes in these diseases.