Abcam, ab247543, Anti-NM23A antibody [EPR3036] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NM23A antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3036,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247543 is the carrier-free version of
ab92327
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NM23A also known as NME1 (non-metastatic cells 1 protein) is a nucleoside diphosphate kinase A. NM23A weighs approximately 17 kDa. This protein is expressed in a variety of tissues with high levels in liver brain and endocrine tissues. By exchanging phosphate groups NM23A is involved in energy transfer in cells. It stabilizes proteins by forming hexameric complexes which are pivotal for its function.
Biological function summary
The NME1 protein plays a significant role in cell proliferation and differentiation. It acts as a marker for the metastatic potential of some tumors where lower expression links to increased metastatic abilities in tumor cells. This protein sometimes works as part of a larger multiprotein complex which influences its functions in cellular processes. It is known for modulating microtubule assembly which affects cell structure and mechanics.
Pathways
NM23A is an important player in signal transduction and DNA repair pathways. It is essential in the regulation of the Ras-MAPK pathway which controls cell growth and differentiation. NM23A interacts with key regulatory proteins such as c-Myc and APC to uphold cellular activities. The protein's influence in these pathways indicates its role in sustaining normal cellular functions and responses.
NM23A relates closely to cancer particularly in metastatic processes and tumor progression. Lower levels of NM23A are associated with the progression of neuroblastoma and breast cancer. In breast cancer NM23A links to the tumor suppressor gene p53 affecting its regulatory functions. Research continues into how NM23A can serve as a useful biomarker for prognosis and therapy in cancer treatment.