Abcam, ab247671, Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GRK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2039(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247671 is the carrier-free version of
ab108502
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRK1 also known as G-protein-coupled receptor kinase 1 or rhodopsin kinase is an enzyme with a molecular mass of approximately 63 kDa. It plays a critical role in the phosphorylation process of activated rhodopsin. This kinase is mainly expressed in the retina particularly in rod photoreceptor cells where it aids in the regulation of phototransduction by rapidly shutting off the rhodopsin signaling cycle.
Biological function summary
GRK1 functions as part of a coordinated mechanism that ensures proper desensitization and recovery of photoreceptors after they are exposed to light. It is instrumental in the phototransduction cascade preventing excessive activation by phosphorylating light-activated rhodopsin which then allows the binding of arrestin. This phosphorylation-arrestin binding step is part of a larger complex that maintains the sensitivity of photoreceptors to changing light conditions.
Pathways
GRK1 participates in the critical visual signal transduction pathway within the retina. It works in conjunction with proteins such as arrestin and rhodopsin to modulate the phototransduction cascade ensuring proper visual responses. This kinase interacts with these proteins to modulate their activity and help in adapting to varying light conditions emphasizing its importance in maintaining visual clarity.
GRK1 mutations are connected to conditions like Oguchi disease a type of congenital stationary night blindness. This disease arises when rhodopsin phosphorylation is disrupted leading to impaired vision adaptation in low light. GRK1 links to the arrestin protein in this context as both proteins are necessary for proper rod cell function and dysfunction in either can lead to similar visual disorders.