Abcam, ab247718, Anti-HDAC10 antibody [EPR3576] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HDAC10 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3576,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247718 is the carrier-free version of
ab108934
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC10 also known as Histone Deacetylase 10 is an enzyme belonging to the HDAC family instrumental in removing acetyl groups from histone proteins and other non-histone proteins. This protein around 71 kDa in mass influences chromatin structure and regulates gene transcription. HDAC10 is expressed in various tissues including the kidney and liver and plays a direct role in the modulation of gene expression through its deacetylase activity impacting cellular processes and environmental responses.
Biological function summary
HDAC10 functions as a regulatory component within the cellular milieu influencing transcriptional repression across several pathways. HDAC10 often forms part of multi-protein complexes although not extensively characterized they include other HDAC family members. It participates in controlling cell growth and cell cycle progress and shows involvement in DNA repair mechanisms. By modulating gene expression through deacetylation HDAC10 impacts cellular proliferation and differentiation.
Pathways
HDAC10 plays a role in the PI3K/AKT signaling pathway which is important for cell survival and growth. HDAC10 interacts with proteins like AKT1 assisting with the transduction of the signaling cascade that contributes to cell cycle progression and apoptosis regulation. Also this enzyme interplays with the autophagy pathway influencing cellular metabolism and homeostasis through its deacetylation functions impacting Beclin-1 regulation and activity levels.
HDAC10 has been implicated in cancer and neurodegenerative diseases. In cancer particularly renal carcinoma HDAC10 may alter oncogene expression and tumor-suppressor levels connecting with proteins such as p53 which are vital in cell-cycle regulation and apoptosis. In neurodegenerative disorders modulations in HDAC10 levels affect huntingtin protein acetylation impacting the progression of diseases like Huntington's disease. Its role in histone and non-histone protein regulation positions HDAC10 as a potential target for therapeutic interventions in these conditions.